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Rf value table
What does a high rf value mean in chromatography.
How to Calculate the Rf Value.
What is RF Value?
In order to calculate the Rf value, we will need to understand the meaning of rf values.
Rf is short for retention or retardation factor.
We can define it as the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin.
Retention factors range between zero and one due to the fact that the solvent front is always larger from the distance traveled by the solute.
Retention factors are useful in comparing the results of one chromatogram to the results of another.
What is rf value in chemistryIf the conditions in which the chromatogram are run are unchanged, the retention factor for a given material should remain constant. This allows unknowns to be compared to the known materials.
Factors affecting Rf values.
The rf values of a particular substance should be constant irregardless of the concentration of the analyte.
However, sometimes the rf value of a substance may change due to the following factors.;
- Concentration of stationary phase
- Mobile phase
- Concentration of mobile phase
- Temperature
- Station
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